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Калкулатор за DSIP

Пресметајте точни волумени за реконституција, инсулински единици и дози по шишенце за секој пептид.

Шишенцето, водата и дозата се познати

DSIPОвој калкулатор е поставен на DSIP

Количество пептид (mg)

BAC вода (ml)

Доза (mcg)

обично 50-500 mcg

mcgmg

Големина на шприцот

Калибрација

U-100U-40

Ознаките се волумен, не активна супстанција

Ознаките мерат волумен, не активна супстанција. 4 IU = 0,04 ml. Колку mg има во нив зависи од смесата. Калкулаторот го пресмета тоа наместо Вас.

Речиси сите инсулински шприцови се U-100. Изберете U-40 само ако на шприцот пишува U-40.

Вашиот резултат · во живо

IUmlОзнаки

Извлечете

4IU

4 IU / 100 IU (0.040 ml)

Концентрација

5.00

mg/ml

Волумен по доза

0.040

Дози по шишенце

дози

по доза

—

DSIP

Клучни податоци

Фреквенција: context-dependent
Вообичаен опсег: 50-500 mcg

Синоними

Delta Sleep-Inducing PeptideEmideltide

Научен профил

DSIP во лексиконот на пептиди: механизам, докази и извори

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What Is DSIP?

DSIP (Delta Sleep-Inducing Peptide) is an endogenous neuropeptide composed of nine amino acids, with the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu. It was discovered in 1977 by the research group of Marcel Monnier and Guido Schoenenberger at the University of Basel. The team isolated the compound from the cerebral venous blood of rabbits in which a deep-sleep state had been induced by electrical stimulation of the thalamus. The name reflects the original observation that the substance could promote delta-wave-dominant sleep — that is, slow-wave deep sleep — in experimental animals.

DSIP occurs naturally in several tissues, including the brain, plasma, cerebrospinal fluid, and the digestive tract. Notably, it is able to cross the blood-brain barrier, which has made it a subject of ongoing neuroendocrine research. Despite its name, DSIP is not a classical sleep medication in the pharmacological sense. The scientific literature tends to describe it as a modulatory peptide that nudges homeostatic processes rather than forcing sedation the way a benzodiazepine or a Z-drug hypnotic does. For this reason, DSIP is most often studied in the context of sleep architecture, stress regulation, and neuroendocrine balance rather than as a pure sleep-onset agent.

The experimental evidence base for DSIP is heterogeneous. Some early studies from the 1980s and 1990s reported effects on sleep patterns, pain perception, stress hormones, and circadian rhythm, while other investigations failed to find consistent results. To date, no approved DSIP drug exists for a defined sleep indication, and large-scale controlled human trials are lacking. DSIP is therefore traded strictly for research purposes. This page outlines the properties of the peptide and the math of reconstituting lyophilized powder, but it does not substitute for scientific or medical advice of any kind.

Mechanisms of Action

Unlike many other peptides, DSIP has no single, clearly defined receptor. Research instead describes a cluster of modulatory effects across several neuroendocrine systems. The following directions of activity are discussed in the preclinical literature:

Sleep architecture: Early animal and human studies suggested DSIP might increase the proportion of slow-wave deep sleep and improve sleep continuity. Unlike sedating hypnotics, DSIP appears to stabilize natural sleep structure rather than override it. These findings, however, are not consistently reproducible and are considered preliminary.
Stress and cortisol modulation: A recurring theme in DSIP research is its influence on the hypothalamic-pituitary-adrenal axis. Several investigations reported a dampening effect on elevated cortisol levels and a normalization of stress-related hormonal responses. This property has led to the hypothesis that DSIP acts less as a sleep trigger and more as a stress buffer.
Neuromodulation and neurotransmitters: DSIP has been associated with effects on various neurotransmitter systems, including serotonergic and GABAergic signaling, as well as on the release of pituitary hormones such as LH and somatoliberin. These broadly distributed effects explain why DSIP is classified in research as a pleiotropic — that is, multi-target — peptide.
Antioxidant and protective effects: Some experimental work described antioxidant properties and a possible protective effect against oxidative stress in neuronal tissue. These too are preclinical observations that have not been confirmed clinically.
Thermoregulation and circadian rhythm: A handful of studies linked DSIP to modulation of body temperature regulation and the internal clock. Because temperature and the sleep-wake cycle are closely coupled, this aspect is discussed in connection with the reported sleep effects.

An important caveat: the mechanisms of DSIP are not fully resolved, and the body of evidence is inconsistent. DSIP should therefore not be regarded as a reliable sleep aid, but as a research molecule whose neuroendocrine effects continue to be investigated.

Dosage Calculation

In the research literature and in usage reports, DSIP is employed in comparatively low microgram quantities. Because there is no approved preparation and no official dosing guideline, the values below are based on published experimental protocols and serve purely to illustrate the arithmetic.

Standard Dosing Parameters

Conservative range: 50–100 mcg (0.05–0.10 mg) per administration
Typical research range: 100–300 mcg (0.10–0.30 mg) per administration
Upper range: 300–500 mcg (0.30–0.50 mg) per administration
Frequency: Context-dependent — many protocols use a single evening dose; some use it only on selected days rather than continuously
Typical syringe: 0.3 mL insulin syringe (29–31 gauge, 6–8 mm needle)

DSIP has a short and variable plasma half-life. Because of its rapid clearance and context-dependent activity, many protocols time the dose close to the desired observation window, such as the evening.

Example Calculation: 5 mg Vial with 2 mL BAC Water

The common vial size is 5 mg. Adding 2 mL of bacteriostatic water gives a concentration of 2.5 mg/mL (2,500 mcg/mL). The volume to draw is calculated with the formula: target dose divided by concentration.

100 mcg dose: 100 ÷ 2500 = 0.04 mL (4 units on a U100 syringe)
200 mcg dose: 200 ÷ 2500 = 0.08 mL (8 units)
300 mcg dose: 300 ÷ 2500 = 0.12 mL (12 units)
500 mcg dose: 500 ÷ 2500 = 0.20 mL (20 units)

At a 200 mcg dose, a 5 mg vial provides 25 administrations. Since DSIP is often used situationally rather than daily, a vial may last considerably longer. Use the DSIP calculator above to determine exact volumes for any vial size, reconstitution volume, and target dose. Because the microgram quantities are so small, precise calculation is especially important: even small misreadings of the syringe correspond to proportionally large dose differences.

Reconstitution

DSIP is supplied as a lyophilized — that is, freeze-dried — powder in sealed vials. Before any use in a research context, it must be reconstituted with bacteriostatic water (BAC water). BAC water contains 0.9% benzyl alcohol, which inhibits microbial growth and extends the usable window of the ready-to-use solution. Sterile water without a preservative is unsuitable for multi-dose vials.

Step-by-Step Reconstitution Protocol

Gather supplies: the DSIP vial, the BAC water vial, alcohol swabs, a sterile draw needle (18–21 gauge), and the 0.3 mL insulin syringe.
Disinfect the rubber septa of both vials with fresh alcohol swabs and allow them to air-dry for about 30 seconds. Do not touch or blow on the septa afterward.
Draw the desired volume of BAC water — typically 2 mL for a 5 mg vial — into the syringe using the draw needle.
Insert the needle into the DSIP vial at an angle so that the tip points toward the inner glass wall rather than directly at the powder cake. A direct stream of water onto the lyophilizate can mechanically stress the peptide.
Slowly push the plunger so the BAC water runs down the inside wall of the glass and gently wets the powder from the side.
Gently roll or swirl the vial between your palms until the powder has dissolved completely. The solution should be clear and colorless. Do not shake or vortex it, as shear forces can damage the peptide.
Label the vial with the reconstitution date and concentration, for example "DSIP 2.5 mg/mL, reconstituted [date]".

If the solution appears cloudy, discolored, or contains visible particulate matter, the vial should be discarded. Because DSIP doses are very small, a correctly calculated concentration is the prerequisite for a reproducible draw-up volume.

Storage and Shelf Life

Lyophilized powder (unreconstituted): Store at −20°C for long-term storage, where the peptide is stable for 24+ months. Short-term storage at 2–8°C in a refrigerator is acceptable for several weeks. Protect from light and moisture, and do not leave it at room temperature for extended periods.
Reconstituted solution: Always store in the refrigerator at 2–8°C. The solution is typically stable for around 28 days. Keep the vial upright and away from light, and discard it after the window has passed regardless of remaining volume.
Do not freeze reconstituted peptide. Freeze-thaw cycles cause protein aggregation and loss of activity. Once reconstituted, the vial must remain refrigerated throughout.
Transport: For travel lasting more than one to two hours, use an insulated cooler with an ice pack. Commercial medication pouches maintain 2–8°C for 24 to 48 hours. Avoid temperatures above 25°C during transport.

Side Effects and Safety Profile

The safety profile of DSIP rests largely on older animal studies and a limited number of small human studies. In those investigations DSIP was generally well tolerated, and severe toxicity was not consistently reported. However, robust long-term human data — for example on continuous use or drug interactions — is entirely absent. Any use therefore remains explicitly within the research domain.

More Commonly Reported Observations

Mild injection-site reactions such as redness, transient swelling, or minor discomfort, which usually resolve within a few hours. Rotating injection sites can reduce localized irritation.
Tiredness, drowsiness, or an altered subjective sense of sleep, particularly when DSIP is used close to the rest period.
Occasionally reported headaches or mild dizziness. These observations are not systematically recorded, and their frequency is unclear.

Rare or Theoretical Concerns

Hormonal modulation: Because DSIP can influence the regulation of pituitary hormones and the stress axis, effects on endocrine balance are theoretically conceivable. Their clinical relevance is unknown.
Interaction with sedating substances: Combination with sleep medications, alcohol, or other centrally depressant agents has not been studied. Additive effects on wakefulness cannot be ruled out.
Heterogeneous evidence base: Because studies on DSIP sometimes yield contradictory results, its side-effect profile is also not fully characterized. The absence of reported harm is not the same as demonstrated safety.

Contraindications

Pregnancy and breastfeeding, as safety data is entirely lacking
Known hypersensitivity to DSIP or any component of the preparation
Existing neurological, endocrine, or psychiatric conditions without specialist medical supervision

At the low research doses described in the literature, no serious adverse events have been systematically documented. Given the thin evidence base and the absence of large controlled trials, caution is warranted, and consultation with a qualified physician is strongly recommended.

Combinations and Stacks

DSIP is sometimes considered alongside other neuropeptides in usage reports because it falls thematically into the domain of stress, recovery, and neuroendocrine balance. The combinations below describe only how research themes complement one another and are not a recommendation for combined use. Mechanisms and evidence bases are not interchangeable.

DSIP and Selank: Stress and Recovery Context

Selank is an anxiolytic-researched neuropeptide associated in the literature with the modulation of anxiety, stress reactivity, and GABAergic signaling. While Selank is discussed in a daytime context around stress, DSIP falls more into the domain of sleep architecture and nighttime recovery. In research, the two are sometimes viewed as complementary components of a broader theme of stress regulation. Their combined effect, however, has not been clinically studied.

DSIP and Semax: Neuroendocrine Research

Semax is an ACTH-derived neuropeptide studied primarily in the context of cognition, attention, and neuroprotection. It thus covers the alert, performance-oriented pole, whereas DSIP sits in the domain of recovery and sleep. Anyone interested in the full spectrum of neuroactive peptides will find the corresponding reconstitution math in the Semax calculator.

DSIP and Epithalon: Circadian Themes

Epithalon is associated in research with the pineal gland, melatonin regulation, and circadian rhythms. Because DSIP has also been discussed in some studies in connection with the internal clock and thermoregulation, the two overlap thematically in the field of chronobiological research. Combined use has not been studied; the comparison is limited to the shared research theme.

An overview of all available calculators can be found on the peptide calculator hub.

Frequently Asked Questions

Is DSIP a sleep medication?

Not in the classical sense. Despite the name Delta Sleep-Inducing Peptide, DSIP does not act like a sedating hypnotic. Research describes it instead as a modulatory peptide that can influence neuroendocrine processes without forcing sedation. The study results regarding its sleep effects are also inconsistent and considered preliminary.

When is DSIP used in research protocols?

Because of its short and variable half-life and its context-dependent activity profile, many published protocols time the dose close to the desired observation window, often in the evening. There is, however, no binding schedule, since there is no approved indication and no official dosing guideline.

How does DSIP differ from melatonin?

Melatonin is a circadian hormone that primarily times the sleep-wake rhythm. DSIP is a neuropeptide with a broader, less clearly defined activity profile that, in research, is linked not only to sleep but also to stress and cortisol regulation. The two substances belong to different compound classes and are not interchangeable.

Does DSIP influence cortisol levels?

Several experimental works reported a dampening effect of DSIP on elevated cortisol values and a modulation of the stress axis. These findings come predominantly from preclinical or small human studies and have not been confirmed by large-scale controlled investigations. A reliable statement about the effect in humans is therefore not possible.

What is the half-life of DSIP?

The plasma half-life of DSIP reported in the literature is short and variable. The peptide is cleared rapidly, which is one explanation for why its effects appear difficult to reproduce and why protocols tie dosing closely to the observation window.

Can DSIP be used intranasally?

The literature describes various routes of administration for DSIP, including intranasal formulations. This page, however, covers only the reconstitution math of the lyophilized powder for subcutaneous-style research workflows. Anyone investigating a different route must adjust concentration and dose accordingly; the underlying formula remains the same.

How long does reconstituted DSIP last?

In the refrigerator at 2–8°C, reconstituted DSIP is typically stable for around 28 days. Lyophilized powder lasts more than 24 months at −20°C. Reconstituted peptide must not be frozen, as freeze-thaw cycles damage the molecule.

Does BergdorfBio offer DSIP?

Yes. DSIP is available from BergdorfBio as a research peptide. You can find the available quantities and variants on the DSIP product page. Use the DSIP calculator on this page to determine the reconstitution and injection volumes for your research protocols.

Medical Disclaimer: The information on this page is provided for educational and research purposes only. DSIP is not an approved drug or medical treatment and is traded strictly for research use. Nothing on this page constitutes medical advice, a diagnosis, or a recommendation to use any specific compound. The scientific evidence base for DSIP is inconsistent, and no large-scale controlled human trials exist. Always consult a qualified healthcare provider before considering any peptide protocol. BergdorfBio assumes no liability for the use or misuse of the information presented here.

Чекор по чекор

Како да реконституирате пептид

Од прашок до готов шприц во пет чекори. Калкулаторот погоре автоматски ги дава количествата.

011
Подгответе BAC вода
Оставете ги шишенцето и водата да ја достигнат собната температура. Пребришете ги двете гумени затворачи со алкохолна газа.
022
Извлечете вода
Извлечете го количеството BAC вода што го препорачува калкулаторот со поголем шприц.
033
Додајте полека
Оставете водата да се слее по внатрешната страна на шишенцето, не прскајте директно врз прашокот.
044
Оставете да се раствори
Вртете го шишенцето нежно, не тресете. Прашокот се раствора бистро во рок од 1-2 минути.
055
Извлечете ја дозата
Извлечете точно до пресметаната ознака со инсулинскиот шприц. Готово.

Референца

Доза и големини на шишенце по пептид

Вообичаени почетни вредности за ориентација. Секој ред го отвора калкулаторот за тој пептид.

Пептид	Категорија	Вообичаена доза	Големини на шишенце	BAC (почеток)	Достапност	Пресметај
VIP	Истражување на невропептиди	-	10 mg	2 ml	Само калкулатор	Пресметај
Ретатрутид (Retatrutide)	GLP-1/GIP/GCG	0,5-12 mg	2 / 5 / 10 / 15 mg	2 ml	Само калкулатор	Пресметај
Кагрилинтид (Cagrilintide)	Аналог на амилин	-	5 mg	2 ml	Само калкулатор	Пресметај
Семаглутид (Semaglutide)	GLP-1	0,25-2,4 mg	2 / 5 / 10 mg	2 ml	Само калкулатор	Пресметај
Тирзепатид (Tirzepatide)	GLP-1/GIP	2,5-15 mg	10 / 15 / 20 / 30 / 60 / 90 mg	2 ml	Само калкулатор	Пресметај
GHK-Cu	Бакарен пептид	1-4 mg	10 / 20 / 50 mg	5 ml	Само калкулатор	Пресметај
AHK-Cu	Бакарен пептид	-	100 mg	5 ml	Само калкулатор	Пресметај
BPC-157	Обнова на ткиво	0,2-0,5 mg	2 / 5 / 10 mg	2 ml	Само калкулатор	Пресметај
TB-500	Тимозин бета-4 (Thymosin Beta-4)	2-5 mg	2 / 5 / 10 mg	2 ml	Само калкулатор	Пресметај
CJC-1295 (без DAC)	GHRH аналог	100-200 mcg	2 / 5 / 10 mg	2 ml	Само калкулатор	Пресметај
Ипаморелин (Ipamorelin)	GHRP	200-300 mcg	2 / 5 mg	2 ml	Само калкулатор	Пресметај
AOD-9604	Губење на маснотии	250-500 mcg	2 / 5 mg	2 ml	Само калкулатор	Пресметај
PT-141	Бремеланотид (Bremelanotide)	-	2 / 5 / 10 mg	2 ml	Само калкулатор	Пресметај
MOTS-c	Митохондриски	5-10 mg	5 / 10 mg	2 ml	Само калкулатор	Пресметај
Селанк (Selank)	Анксиолитик	150-500 mcg	2 / 5 / 10 mg	2 ml	Само калкулатор	Пресметај
SS-31	Еламипретид (Elamipretide)	0,1-1 mg	5 / 10 mg	2 ml	Само калкулатор	Пресметај
Glow Stack	Stack	0,5-2 mg	70 mg	3 ml	Само калкулатор	Пресметај
Klow Stack	Stack	0,5-2 mg	55 mg	3 ml	Само калкулатор	Пресметај
Меланотан II (Melanotan II)	Меланокортин	100-1000 mcg	5 / 10 mg	2 ml	Само калкулатор	Пресметај
Меланотан I (Melanotan I)	Меланокортин	-	5 / 10 mg	2 ml	Само калкулатор	Пресметај
Серморелин (Sermorelin)	GHRH аналог	50-300 mcg	5 mg	2 ml	Само калкулатор	Пресметај
Тезаморелин (Tesamorelin)	GHRH аналог	1-2 mg	10 / 20 mg	2 ml	Само калкулатор	Пресметај
Тимозин алфа-1 (Thymosin Alpha-1)	Тимусен пептид	0,8-3,2 mg	1.6 / 5 / 10 mg	1 ml	Само калкулатор	Пресметај
Епиталон (Epithalon)	Долговечност	2-10 mg	10 / 20 / 50 mg	2 ml	Само калкулатор	Пресметај
KPV	Имун систем / бариера	100-1000 mcg	5 / 10 mg	2 ml	Само калкулатор	Пресметај
HGH фрагмент 176-191	Метаболичен фрагмент	100-500 mcg	2 / 5 / 10 mg	2 ml	Само калкулатор	Пресметај
Семакс (Semax)	Когнитивно	100-1000 mcg	10 / 30 mg	2 ml	Само калкулатор	Пресметај
N-ацетил Семакс (N-Acetyl Semax)	Когнитивно	100-1000 mcg	5 / 10 mg	2 ml	Само калкулатор	Пресметај
Хексарелин (Hexarelin)	GHRP	50-200 mcg	2 / 5 mg	2 ml	Само калкулатор	Пресметај
GHRP-6	GHRP	50-300 mcg	2 / 5 mg	2 ml	Само калкулатор	Пресметај
GHRP-2	GHRP	50-250 mcg	2 / 5 mg	2 ml	Само калкулатор	Пресметај
DSIP	Сон	50-500 mcg	10 mg	2 ml	Само калкулатор	Пресметај
5-Amino-1MQ	Метаболичен	2,5-10 mg	10 mg	2 ml	Само калкулатор	Пресметај
Пинеалон (Pinealon)	Истражувачки пептид	-	10 mg	2 ml	Само калкулатор	Пресметај
Сурводутид (Survodutide)	GLP-1/глукагон	-	10 mg	2 ml	Само калкулатор	Пресметај
Киспептин-10 (Kisspeptin-10)	KISS1R / GPR54	-	10 mg	2 ml	Само калкулатор	Пресметај
HGH 191AA	Хормон на раст	1-5 mg	10 mg	1 ml	Само калкулатор	Пресметај
NAD+	Долговечност / кофактор	100-500 mg	1000 mg	10 ml	Само калкулатор	Пресметај

Вредностите служат за ориентација во истражувачки контекст и не заменуваат стручен совет.

Зошто е важна прецизната доза на пептидот

Точната пресметка на концентрацијата и волуменот за инјектирање изгледа едноставна, но е најчест извор на грешки. Дури и 10 % грешка при реконституцијата значи или премалку активна супстанција или ненамерно преголема доза.

Лиофилизираните пептиди се испорачуваат во милиграми и се раствораат во бактериостатска вода (BAC вода). Односот пептид-вода ја определува концентрацијата, а од неа зависи колку ознаки извлекувате со инсулинскиот шприц. Инсулинските шприцови се поделени по ознаки, не по милиграми. Калкулаторот ја прави оваа конверзија, вклучително проверката на веројатноста против најопасната грешка: мешање на mg и mcg.

Често поставувани прашања

Која е разликата меѓу mcg и mg?

Милиграмите (mg) се единицата во која се продаваат лиофилизираните пептиди, на пример „5 mg BPC-157“. Микрограмите (mcg) ја мерат поединечната доза, на пример „250 mcg по инјекција“. 1 mg = 1.000 mcg. Калкулаторот конвертира автоматски и предупредува кога внесот изгледа како замена.

Што значи „U-100“ на инсулинскиот шприц?

U-100 значи дека шприцот е калибриран на 100 единици по милилитар. 100 IU = 1 ml, значи 10 IU = 0,10 ml, без оглед на тоа што има во шприцот. Пресметката на IU се базира токму на овој однос.

Зошто калкулаторот понекогаш прикажува многу мали волумени?

Со многу пептид и малку вода растворот станува многу концентриран, па волуменот по доза е ситен и тежок за читање. Користете го помошникот за растворање: тој го наоѓа волуменот вода со кој се добива чиста и лесно читлива ознака.

Колку трае реконституираниот пептид?

Во фрижидер на 2-8 °C обично 21-30 дена. Означете го шишенцето со датумот на реконституција. Заматени раствори или честички не се прифатливи.

Како функционира калкулаторот за смеси како Glow Stack?

Смесите содржат неколку активни супстанции во едно шишенце. Едноставно изберете ја смесата како Ваш пептид: калкулаторот дополнително покажува колку од секоја компонента има во Вашата инјекција. За комбинации од неколку одделни шишенца користете го Составувачот на комбинации.

Морам ли секој пат одново да го пополнувам калкулаторот?

Не. Последните поставки се чуваат локално во прелистувачот и се враќаат при следната посета. Можете и да вратите пресметки од историјата, да ги зачувате како протокол или да ги споделите преку линк.

Поврзани пептиди

Селанк (Selank)Анксиолитик Семакс (Semax)Когнитивно Епиталон (Epithalon)Долговечност

Сите калкулатори

Изберете пептид

Изберете пептид за да ја отворите неговата калкулаторска страница со пополнети вредности.

Само калкулатор